|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. dimB is required for stalk cell induction and repression of
non-vacuolar cell death in response to DIF-1. (A) Repression of
spore cell formation by DIF-1 was measured in an 8-Br-cAMP assay. In the
absence of DIF-1, all strains produced similar numbers of spores. Only
dimA- mutant cells produced spore cells in the presence of
DIF-1. Each assay was performed at least three times and the results of a
representative assay are shown. dimAB- represents the
double mutant dimA-dimB- and AX4 is the wild
type. (B) dimB- cells were stained with DAPI
without DIF-1 treatment (a,b) or after DIF-1 treatment (c,d). Micrographs were
taken with phase contrast (a,c) or fluorescence microscopy (b,d). (C)
dimB- mutants produce cells with identical characteristics
to those that have undergone non-vacuolar cell death. (a) Phase-contrast image
showing cell morphology. Cells appear rounded and contain a dense/granular
region. (b) Staining of DNA with DAPI. (c) F-actin distribution detected with
phalloidin. (D) Model of DIF-1 responses based on the epistatic
relationship between DimA and DimB in the 8-Br-cAMP assay.
|
