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Fig. 2. Cni protein localization. (A) Myc-tagged Cni (Cni-myc)
expressed from the endogenous promotor accumulates in the oocyte during early
and middle oogenesis (white arrows). At stage 10, strong expression in the
nurse cells indicates maternal loading (arrowhead). The protein is also
detectable in the somatic follicular epithelium (black arrows). Right panel,
magnification of boxed area. (B) C-terminally myc-tagged Cni protein
(Cni-myc, red) expressed in the squamous follicle epithelium forms discrete
punctate structures, that are often associated (arrows) but not necessarily
tightly colocalized with the large reticulate ER of these cells labelled with
PDI-GFP (green). (C) Cni-myc (red) does in most cases (arrows) not
colocalize with the punctate and disperse Golgi apparatus of the same cells,
marked by the p120 Golgi protein (blue). (D) Cni-myc containing
structures (red) are largely co-stained (arrows) with an antibody against the
KDEL receptor (blue). This protein cycles between ER and Golgi. The total pool
of KDEL-receptor positive structures exceeds that co-staining with Cni-myc.
(B-D) DNA (DAPI) is shown in light blue. Scale bars: 10 µm.
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