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Fig. 8. Gal4-mediated Grk overexpression in the germline can overcome the secretion block in cni ovaries. (A) Wild-type egg chamber. Endogenous Grk (red) is tightly localized to the dorsal anterior corner of the oocyte. Cells are outlined with Phalloidin (green). (B) Wild-type egg. The dorsalmost chorion structure is the operculum (op). Dorsal appendages (da) are derived from more lateral positions. (C,D) Grk overexpression in a wild-type background using the maternal tubulin:Gal4-VP16 driver. (C) Despite a massive increase in the amount of Grk protein, there is still a Grk gradient within the oocyte with highest levels close to the oocyte nucleus. (D) The eggs maintain DV polarity although they have an expanded operculum (op) and the dorsal appendages (da) are shifted to the ventral side. (E-G) Overexpression of Grk in a cni^AR55/Df(2R)H60 background using the maternal tubulin:Gal4-VP16 driver line. (E) High amounts of Grk protein are evenly distributed within the oocyte. (F) The eggs lack DV polarity, but the presence of dorsal appendage material (da) and the suppression of a posterior micropyle indicate low levels of Grk signalling. (G) Despite formation of dorsal appendage material (da), 30% of the eggs lack anteroposterior polarity as can be seen from the presence of a posterior micropyle (m). This indicates a lack of temporal control of Grk signalling.

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