|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Differential localization of Hh-GFP, HhN-GFP and Hhc85s-GFP in the
plasma membrane. (A-C) Extracellular labeling of Hh-GFP (A),
HhN-GFP (B) and Hhc85s-GFP (C) showing their distribution at the apical versus
basolateral plane. (a-c) AP z sections of the same wing discs in A-C.
(a') AP z sections of a wing disc double-stained with anti-Hh
antibody (green) and anti-DE-cadherin (purple) as an apical region marker. The
three Hh forms occur in the extracellular space on the apical side of the
producing cells (asterisk), while only lipid-unmodified forms of Hh appear in
the apical extracellular space in the A compartment (arrows in apical planes
and Z sections). Wild-type Hh forms an extracellular gradient in the
basolateral A cells next to the AP compartment, while the lipid-unmodified
forms are poorly represented and do not form a gradient (arrowheads in
basolateral panels and z sections).
|
