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Fig. 5. Inductive effect of HhN but not of Hh from peripodial cells upon disc
proper cells. (A-C') Expression of Hh-GFP
(A,a,A',a'), HhN-GFP (B,b,B',b') and Hhc85s-GFP
(C,c,C',c') in peripodial cells using the Ubx-Gal4
driver. The Ubx is expressed at high levels in the part of the peripodial
membrane corresponding to the P compartment (arrowheads in A,B,C). HhN-GFP and
Hhc85s-GFP, but not wild-type Hh-GFP, are observed in internalized vesicles in
the cells of the disc proper (arrows in posterior and arrowheads in anterior
compartment in A',B',C'). The internalized vesicles of
HhN-GFP are more clearly visualized in the A than in the P compartment, unlike
Hhc85s-GFP vesicles, which are visible in both A and P compartments. Dpp (red)
is ectopically induced in the disc proper cells in HhN-GFP/Ubx-Gal4
disc (B,B'). (D-G) Wing and notum phenotypes of the
Hh-GFP/Ubx-Gal4 and Hhc85s-GFP/Ubx-Gal4 (D,F),
HhN-GFP/Ubx-Gal4 flies (E,G). The notum is enlarged and contains
extra-macrochaetae (G) and there is modification of the wing pattern (E) in
HhN-GFP/Ubx-Gal4 flies. (H) Diagrams showing the organization
of the disc proper cells and peripodial cells in a front- and transverse view
of a wing imaginal disc.
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