|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Diffusion properties and signaling activity of Hh-GFP, HhN-GFP and
Hhc85s-GFP in ttv- mutant cells. (A-K)
ttv- clones labeled by the absence of b-gal staining
(blue) in wing discs expressing Hh-GFP (A-C), HhN-GFP (E-G) and Hhc85s-GFP
(I-K) with the hh-Gal4 driver (green) and stained for Ptc (A,E,I),
Col (B,F,J) and Iro (C,G,K) in red. HhN-GFP and Hhc85s-GFP, but not wild-type
Hh-GFP, appear inside the ttv- cells and in the cells
located anterior to the clone (arrows in E,I). Ptc and Col are expressed only
in the first row of ttv- cells next to the AP border
(arrowheads in A,B,E,F,I,J) and Hh-GFP vesicles co-localize with Ptc in all
cases (arrowheads in A). Iro is expressed only in the first row of cells in
the wild-type Hh-GFP- and Hhc85s-GFP-expressing discs (arrows in C,K). In
HhN-GFP-expressing discs, Iro is activated throughout the
ttv- clone (arrowheads in G, clone 1 in H). However, Iro
expression decreases in a clone far from the HhN-GFP-producing cells because
HhN-GFP levels are lower than at the AP compartment border (arrow in G, clone
2 in H). (D,H,L) Graphs showing the activation of Ptc, Col and Iro in
ttv- clones abutting the AP compartment border (clone 1)
or located distant to the AP border (clone 2) by wild-type Hh-GFP (D), HhN-GFP
(H) and Hhc85s-GFP (L). The light green represents Hh levels and the graded
distribution of the protein produced in the P compartment and secreted to the
A compartment; the dark green represents the signaling activity induced by the
different Hh forms. The broken lines represent the Hh gradients and signaling
activity in a disc that does not contain ttv- clones.
|
