DEV02211 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Fig. S1. Neuroblast rotation in vitro does not account for variability in spindle orientation. (A-C) Series of still images from a time-lapse DIC movie from an isolated neuroblast, showing interphase and telophase panels during three consecutive cell cycles. Position of the same Concavalin A-coated bead labeled in red (A), yellow (B) or green (C) is mostly restricted to a single neuroblast quadrant throughout three cell cycles, independent of the position of the newly born GMC. (D) Summary of A-C, showing no correlation between bead position and GMC position. Bead position was visually identified every 5 seconds and marked with colored spheres in cartoon summaries below A-C every 60 seconds. Straight line between colored spheres marks pathway traveled between time points. s, bead position one minute after nuclear envelope reforms following cytokinesis; f, bead position 1 minute before the neuroblast nuclear envelope reforms at the end of cytokinesis; NEB, nuclear envelope breakdown. Scale bar: 10 mm.

• Movie 1 - Movie 1. Confocal movie of a clustered neuroblast from G147-GFP embryos.

• Movie 2 - Movie 2. Confocal movie of an isolated neuroblast from G147-GFP embryos. White asterisk indicates the neuroblast centrosomes position prior to mitotic entry. Red arrow indicates towards the apical spindle pole.