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Fig. 2. Extrinsic cues orient the neuroblast division axis over multiple cell
cycles. (A) Series of still images from a time-lapse DIC movie
showing six consecutive telophase figures from a single clustered or
(B) isolated neuroblast in primary cell culture. The red arrow points
towards the developing GMC along the neuroblast intrinsic apicobasal polarity
axis. (A,B) Diagrams on the right summarize GMC position at telophase from all
six consecutive divisions, showing tight grouping of GMCs in clustered
neuroblasts versus dispersal of GMCs in isolated neuroblasts. Maximum distance
between two sister GMCs is uncovered by a 53° angle in clustered
neuroblasts and 141° angle in isolated neuroblasts. (C)
Quantitation of GMC position at telophase during successive divisions in
clustered and isolated neuroblasts. Only neuroblasts dividing more than three
times were scored. (D) Three serial confocal sections of a cell cluster
fixed and stained for neuroblast and epithelial markers (as indicated). The
Insc-positive neuroblast cortex directly contacts several DE-cadherin-positive
epithelial cells. Brackets indicate the same neuroblast in the three sections,
asterisks indicate the DE-cad positive epithelium only in the left panel and
arrows indicate localized DE-cadherin in the middle and right panels. Scale
bar: 10 µm.
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