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Fig. 4. In the presence of extrinsic cues, spindle orientation correlates with
G2 centrosome position despite centrosome rotation at prophase. (A-F)
Series of still images from two different confocal time-lapse movies from
clustered neuroblasts from a single division, dissociated from G147-GFP
embryos. Mitotic stages labeled above each panel. (A-C) An example of a
neuroblast showing centrosome rotation prior to spindle formation and
(D-F) an example of a neuroblast showing no centrosome rotation prior
to spindle formation. A red line connects both apical and basal centrosomes
during mitosis or the G2 centrosomes and approximate cell center during
interphase. Brackets indicate neuroblasts. The white arrows indicate the two
centrosomes that remain closely apposed at G2. Scale bar: 10 µm.
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