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Fig. 5. In the absence of extrinsic cues, centrosome position and the cell
division axis drift over multiple cell cycles. (A-C) Series of
still images from three different time-lapse confocal movies of isolated
neuroblasts dissociated from G147-GFP embryos. Mitotic stages are labeled
above each panel. A red line connects both apical and basal centrosomes during
mitosis through the spindle axis or the G2 centrosomes and approximate cell
center during interphase. The white arrow indicates the two centrosomes that
remain closely apposed at G2. (D-G) Quantitation of the angle between
the following two lines: (D) a line connecting the G2 centrosomes to cell
center and a line through the metaphase spindle as seen in A; (E) a line
through the metaphase spindle and a line through the telophase spindle as seen
in A; (F) a line through the telophase spindle and a line connecting the G2
centrosomes to cell center in the subsequent division as seen in B; (G) a line
connecting the G2 centrosomes to cell center and a line connecting the G2
centrosomes to cell center in the following division as seen in C. Gray bars
indicate neuroblast percent. Scale bar: 10 µm.
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