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Fig. 2. Expression analysis of Cullin-1. (A) RNA expression
pattern of Cullin-1 during early X. tropicalis development.
Cullin-1 is ubiquitous at blastula (1, lateral view) and gastrula (2,
vegetal, 3, animal view) stages. At neurula stages (4, anterior view) it
becomes enriched in neural tissue. At tadpole stages, it becomes enriched in
the neural tube, eye and branchial arches (5+6, anterior towards the left,
dorsal upwards). (B) Cullin-1 protein presence during development.
Westerns with an anti-Cullin-1 antibody were carried out on protein extracts
of whole X. laevis embryos at different developmental stages.
Cullin-1 protein is found throughout development. Cullin-1 protein levels are
slightly increased during late neurula/early tadpole stages.
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