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Fig. 5. Comparative dose response of Cul1-C75 and Cullin-1 in
inducing developmental abnormalities. Embryos were injected with different
doses of Cul1-C75 or Cullin-1 RNA animally into one
blastomere at two-cell stage and grown to tadpole stages (stage 39/40).
Morphological analysis was carried out by comparing the uninjected with the
injected side and embryos were scored for defects. The phenotype predominantly
consisted of ectopic melanocytes, ectopic tissue (such as epidermal folds),
eye defects and secondary axes. The most robust phenotype was ectopic
melanocytes, which could be seen in almost all of the injected embryos. The
dose of RNA injected correlates with the penetrance of the phenotype. The
truncated Cullin-1 construct (Cul1-C75) was more potent at
causing a phenotype than the full-length construct.
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