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Fig. 6. Phenotypes observed in Cul1-C75 and Cullin-1 injected
embryos. (A) Wild-type embryo for comparison. The most striking
phenotype observed was an increase in melanocytes (B), but eye defects
(C, star represents where the eye should be) and secondary axes
(D) were also commonly observed (see
Fig. 5 for quantification).
Embryos were hybridised in situ with a probe for N-tubulin, a marker
for differentiated neurons (purple). Ectopic N-tubulin can be
observed in the secondary axis (D). The lineage tracer lacZ
(light-blue staining) indicates the area of injection. Lateral view with
anterior towards the left and dorsal towards the top. The injected side is
shown. Transverse sections of the embryos show clearly the large upregulation
of melanocytes (E-G) and the ectopic tissue (white arrowhead) in the
injected side. Neural differentiation in the neural tube is not affected (as
marked by N-tubulin); however, cranial ganglia neurons
(N-tubulin positive, arrows) are absent (compare B,C with A and E,F
with control side). Transverse view with dorsal towards the top and the
injected side on the right.
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