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Fig. 7. Phenotype of a severely C-terminally truncated form of Cullin1,
Cul1-C477. In order to ensure that the C-terminally truncated
Cul1-C75 does not act as wild-type form of Cullin-1, we made a
Cullin-1 construct (Cul1-C477) that is lacking all of the C
terminus (Fig. 1B). A 250 pg
dose of this construct together with the lineage tracer lacZ (light
blue) were injected animally at the two-cell stage into X. laevis
embryos. Embryos were analysed at tadpole stages for morphological defects.
Injected embryos (A-C,E) showed an accumulation of melanocytes (A-C,
arrow in C), secondary axes (B) and ectopic tissue (C), all phenotypes
previously observed with the other Cullin-1 constructs. For
comparison, uninjected control embryos are shown (D,F). This indicates
that all of these constructs act in the same manner, which is most likely to
be a dominant-negative mechanism. All embryos are shown laterally. In A-D,
anterior is towards the left; in E,F, embryos are facing each other.
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