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Figure 1


Fig. 1. The follicle sets oocyte pHi during growth. (A) An example of one experiment in which follicle-enclosed and denuded oocytes were microinjected with SNARF-dex to determine pHi. Brightfield (left) and fluorescence (right) images are shown. Note that fluorescence is restricted to the oocyte. Scale bar 50 µm. (B) Oocytes within each experiment were grouped in 5 µm increments. pHi increased significantly with increasing size in denuded oocytes (P<0.0001), whereas there is no significant difference in pHi between size groupings in follicle-enclosed oocytes (P>0.25). Each point comprises between four and 17 oocytes from two to eight replicates.





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