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Figure 2


Fig. 2. The follicle affords the growing oocyte HCO3-/Cl- activity. (A) pHi was monitored in denuded (top row) and follicle-enclosed (bottom row) oocytes simultaneously. Cl- was removed from the bathing medium at 10 minutes in each experiment. Traces shown are the mean of all experiments performed. Note that Cl- removal causes sharp increases in pHi in all follicle-enclosed oocytes, but only in larger denuded oocytes. (B) Plot of the rate of pHi increase, which provides an indication of HCO3-/Cl- activity. HCO3-/Cl- exchange rate increases significantly with increasing oocyte size in denuded oocytes (P<0.0001), whereas there is no difference between sizes in follicle-enclosed oocytes (P>0.8). DIDS inhibited Cl--induced pHi increases in follicle-enclosed oocytes (A, bottom row, far right). Each point comprises between four and 17 oocytes from two to eight replicates.





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