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Figure 3


Fig. 3. Granulosa cells influence oocyte pHi in cumulus-oocyte complexes. (A) Example of an experiment in which cumulus-enclosed and denuded oocytes were compared with SNARF-dextran. Brightfield (left) and fluorescence (right) images are shown. (B) Cl- was removed from the bath at 10 minutes. (C,D) Cumulus-enclosed oocytes exhibited a significantly higher resting pHi (C; P<0.05) and rate of pHi increase (D; P<0.01) than did denuded oocytes. Data are from four replicates. Total oocytes: denuded, n=25; cumulus-enclosed, n=23.





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