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Fig. 3. The dauer lethality in aak-2(rr48) mutants is not due to a germ
line defect. Precursors of the germ line (Z2, Z3), and/or of the somatic
gonad (Z1, Z4), were ablated using laser microsurgery. Larvae were
subsequently plated at 25°C and assayed for dauer lifespan. The success of
the ablation was monitored 24 hours after surgery, by examining
lag-2::GFP expression.