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Figure 1


Fig. 1. Characterization of uri-1 in C. elegans. (A) Genomic structure of C. elegans uri-1 (C55B7.5) and uri-1 deletion mutant (tm939). URI-1 encodes a 446 amino acid protein with a putative prefoldin domain (PFD), a direct binding site for the common subunit of all three RNA polymerases RPB5 (RPB5) and the URI-1 box depicted below. (B) Images from wild-type adult hermaphrodites, uri-1(RNAi)F1 and homozygous uri-1(tm939) adult hermaphrodites raised at 25°C and stained with DAPI. Arrows indicate haploid sperm and arrowheads indicate spermatids. Anterior is towards the left and dorsal is towards the top throughout. Scale bar 10 µm. (C) Northern blot was performed as described in the Materials and methods and shows that uri-1 is a germline-enriched transcript expressed in both types of gametes. Ribosomal RNA was used as loading control.





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