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Fig. 3. Loss of URI-1 blocks cell cycle progression in the C. elegans
germline. (A) (Left) Quantification of G2/M phase cells in
untreated wild-type (n=35, dark) and uri-1(RNAi)F1
(n=35, grey) synchronized adult hermaphrodites
(*P=5.2x10-5). Error bars represent s.d.
(Right) DAPI and PH3 staining of the distal part of the germline of a
uri-1(RNAi)F1 and a wild-type worm. The arrowheads indicate mitotic
germline cells with apparently condensed chromosomes and different extent of
enlargement. This DNA morphology accumulates in germlines upon loss of
uri-1 function and resembles prometaphases which are not recognized
by the PH3 antibody (Cy3). Scale bar: 10 µm. (B) (Right) Staining
with anti-phospho-histone H3 antibody (Cy3) of germ cell nuclei (visualized
with DAPI) in the distal region of the gonad of the indicated strains. (Left)
PH3-positive nuclei were counted per gonad arm in synchronized adults
uri-1(tm939) homozygous (n=24), uri-1(RNAi)F1
(n=65) and wild type (n=65, Student's test
*P=7.9x10-8,
#P=1.0x10-4). Scale bar 10 µm. Error
bars indicate s. d. (C) Anti-PH3 (Cy3) and DAPI staining of a
homozygous uri-1(lf) mutant and wild-type gonads. Arrowheads indicate
sperm. Scale bar: 10 µm. Anterior is towards the left and dorsal is towards
the top.
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