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Fig. 6. Analysis of hypaxial cell migration in XHas2-Mo and
XCD44-Mo injected embryos. (A,A') Lateral view of
control (A) and injected (A') side of an XHas2-Mo injected
embryo (mild phenotype) at stage 37 showing the relative position of
differentiating hypaxial muscle cells, as highlighted by 12/101 antibody
staining (arrows). (B,B') Transversally sectioned XHas2-Mo
injected embryo hybridized with CA and coloured with Hoechst to
visualize the nuclei, in which it is evident that the somite structure is
altered and the ventrolateral migration of hypaxial muscle cells is impaired
(red arrow). (C) Lateral view of the control side of a
XHas2-Mo-injected embryo analyzed for XPax3 expression by in
situ hybridization at stage 37 highlighting hypaxial muscle cells migrating
ventrally (arrow). (C') Injected side of the embryo shown in C,
in which hypaxial muscle cells are blocked at the ventral aspect of somites
(red arrow). (D,D') Stage 37 XCD44-Mo injected embryos
immunostained with the 12/101 antibody. Although the trunk musculature appears
unaffected by XCD44 loss, migration of hypaxial cells is greatly
reduced. (D) Control side of the embryo; (D') injected side. (E-G)
Ventral views of stage 43 embryos immunostained with the 12/101 antibody that
show the final position of the ventral body wall musculature in uninjected
(E), XCD44-Mo injected embryo (F) and XHas2-Mo injected
embryo (G). The side of injection is visualized by X-gal staining in blue and
indicated by the arrow. (E'-G') Lateral views of the
same embryos shown in E-G, respectively.
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