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Figure 2


Fig. 2. Transcription of egl-1 correlates with programmed cell death. Nomarski optics (A,C,E) and epifluorescence (B,D,F) of the posterior ventral nerve cord of L3 stage larvae carrying an integrated Pegl-1histone:gfp reporter construct localized to nuclei. The descendants of P11.aaa are reproducibly located immediately posterior to the hypodermal cell P10.p. (A,B) In ced-3; Pegl-1histone:gfp transgenic animals (30 out of 30 animals), egl-1 is expressed in the two cells that undergo programmed cell death in the P11 lineage (P11.aap and P11.aaap). (C,D) In mab-5(n1384); ced-3; Pegl-1histone:gfp transgenic mutants, the egl-1 transcriptional reporter is not expressed (27 of 30 mutants) in P11.aaap, which survives in mab-5 mutants. (E,F) In ceh-20(ay42); ced-3; Pegl-1histone:gfp mutants, the egl-1 reporter is not expressed (58 out of 60 mutants) in P11.aaap, which survives in mab-5 and ceh-20 mutants. The P12 lineage descendants arise in the preanal ganglion, which contains other neuronal cells; consequently, we were unable to identify unambiguously P12.aaap. However, the Pegl-1histone:gfp reporter was expressed in two rather than three cells in the preanal ganglion of mab-5 and ceh-20 mutants.





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