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Fig. 1. Expression of chick Nkx3.2 is restricted to immature proliferative
chondrocytes during endochondral bone development. Chick embryos, stages
as indicated, were pulse labeled with BrdU before harvesting and processing
for section in situ hybridization (ISH; A-J) or stained with an
anti-BrdU antibody (K,L). Arrowheads indicate approximate
boundary of BrdU-positive zone in parallel sections. Arrows (A-F) indicate
distal-most boundary of cartilage, marked by col IX and Sox9 expression.