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Figure 4


Fig. 4. Nkx3.2-{Delta}C-VP16 acts as a reverse function form of Nkx3.2 and accelerates chondrocyte maturation. The right wing buds of day 3 chick embryos were infected with RCAS(A)-Nkx3.2-{Delta}C-VP16 virus. Embryos were harvested at either E10 (A,C) or E8.5 (B), and processed for Alcian Blue/Alizarin Red staining (A) or section ISH (B,C). The extent of mineralized bone (red bar in A) is increased in limbs infected with RCAS-Nkx3.2-{Delta}C-VP16 relative to the contralateral control limb. Double-headed arrows (B,C) compare either the peripheral-most extent of col X expression or the peripheral-most extent lacking col II expression.





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