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Figure 4


Fig. 4. Posterior truncations and tail bud development. (A-C) Bright-field photographs of E13.5 littermates (caudal region). (D,E) Hematoxylin and Eosin stained sections through kidneys of wild-type and severely truncated Fgfr1{Delta}Frs/{Delta}Frs embryos; bar indicates relative magnification. (F) T (Brachyury) and (G,H) Wnt3a whole-mount in situ hybridization. (I) Quantitation of proliferation (pH3, P=0.33) and apoptosis (TUNEL, P=0.09) in E10.5 tail bud sections. Each data point represents the mean of three to eight sections through each of at least three embryos. Error bars indicate ±1 s.d.; P values were determined by Student's t-test.





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