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Figure 5


Fig. 5. Pharyngeal arch (PA) development. (A-C) Phosphohistone H3 immunostained PA sections (E10.5). PA1 is labeled, arrowheads indicate PA2. (D,E) Fgf8 whole-mount in situ hybridization (PA region); arrows indicate PA2 Fgf8 expression domain lost in mutant embryos. (F-H) Migrating NCCs in the pharyngeal region of stage-matched embryos, visualized by Sox10 in situ hybridization. Arrows indicate limits of NCC migration into PA1, 2. (I-L) Nile Blue staining of cell death in the pharyngeal region (E10.5). I/J and K/L are stage-matched control/mutant embryo pairs.





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