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Figure 6


Fig. 6 . Inhibition of Ena/VASP results in defects in somite rotation and boundary formation. Dorsal explants of stage 25 embryos expressing FP4-mito (A-C) or AP4-mito (D-F) were coimmunostained for Xena (A,D; green in C,F) and ß1-integrin (B,E; red in C,F). (A-C) FP4-mito expression leads to mis-localization of Xena that is accompanied by the presence of misoriented cells within somites and disruption of ß1-integrin localization at intersomitic boundaries. (D-F)AP4-mito expression does not alter Xena or ß1-integrin localization, and is accompanied by normal somite formation. Scale bar: 100 µm.





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