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Figure 1


Fig. 1. CDK-1 depletion impairs oocyte maturation. (A-F) Wild-type (WT; A,C,E) and CDK-1-depleted (B,D,F) animals stained with pH3 antibodies (A,B), NOP1 antibodies (C,D), and TOTO-3 to visualize DNA (E,F). Scale bar: 20 µm. (G) Quantitation of pH3- and NOP1-positive nuclei by oocyte position in the proximal gonad. In between the graphs is a table showing the number of oocytes analyzed. N2 is the wild-type strain. (H) DIC image of one-cell arrested embryos in the uterus of a CDK-1-depleted adult. (I) One-cell arrested embryos dissected from the uterus of a histone H2B::GFP transgenic animal depleted of CDK-1. The four embryos marked by arrows show the congression of the oocyte chromosomes, similar to metaphase of meiosis I. Older embryos remain arrested as one-cell embryos but their chromosomes have decondensed. spth, spermatheca.





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