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Fig. 2. WEE-1.3 depletion causes precocious oocyte maturation. (A-F)
Wild-type (A,C,E) and WEE-1.3-depleted (B,D,F) animals stained with pH3
antibodies (A,B), NOP1 antibodies (C,D), and TOTO-3 (E,F). Scale bar: 20
µm. (G) Quantitation of pH3- and NOP1-positive nuclei by oocyte
position in the proximal gonad. Below each graph is a table showing the number
of oocytes analyzed. (H) Quantitation of the presence of an intact NE
in the -1 oocyte from adult hermaphrodites untreated (WT) or depleted of
WEE-1.3 or WEE-1.1 (control). Animals were analyzed 16-20 hours
post-injection. n, number of gonad arms analyzed.
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