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Fig. 6. WEE-1.3 depletion results in the appearance of aberrant microtubules in
developing oocytes. (A-E) Live 
-tubulin::GFP animals were
subjected to wee-1.3 RNAi. (A,B) Untreated animals. (A) Oocytes with
typical cytoplasmic tubulin cytoskeletons. (B) A wild-type meiotic spindle;
such a structure is normally observed in the oocyte upon fertilization in the
spermatheca, or in the +1 embryo in the uterus, and is never observed in
WEE-1.3-depleted oocytes in the spermatheca or uterus. (C-E) WEE-1.3-depleted
oocytes in the oviduct (C,D) or the uterus (E). White arrows mark the
perinuclear tubulin foci. Oocytes in D are outlined in black. (E) Tubulin
clouds (marked by carets) form around the coalesced chromosomes of
WEE-1.3-depleted oocytes that fail to be fertilized but nonetheless end up in
the uterus. The spermatheca is to the right in each gonad shown. Scale bars:
in A, 20 µm for A,C-E; 10 µm in B. (F) Quantitation of the number
of oocytes with tubulin foci scored by oocyte position in the proximal gonad.
spth, spermatheca.
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