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Fig. 7. Oocyte chromosomes do not maintain a diakinetic arrangement upon WEE-1.3
depletion. (A-F) Wild-type (A,B), WEE-1.3-depleted (C-E), and
emo-1 (F) animals were DAPI stained to visualize oocyte chromosomes.
Arrows and white boxes mark the normal diakinetic arrangement of chromosomes
in growing oocytes; those marked with a white box are enlarged in B,D. White
lines mark the spermatheca and the highly condensed sperm chromosomes. Carets
(^) in C mark the most proximal oocyte chromosomes that have coalesced. (E)
The -3 oocyte has stringy chromosomes and is flanked by normal diakinetic
oocytes to the right and oocytes with coalesced chromosomes to the left.
Asterisk in F marks an endoreplicating oocyte. (G-M) Images of
live, untreated (G-J) or WEE-1.3-depleted (K-M) histone H2B::GFP transgenic
animals. Diakinetic chromosomes are apparent in wild-type (G-I) and
WEE-1.3-depleted (K) animals, and are marked with arrows and white boxes; the
one in the white box in G is enlarged in H. The chromosomes of a wild-type
fertilized oocyte congress during metaphase I (marked by a circle in I and
enlarged in J). The coalesced chromosomes of WEE-1.3-depleted oocytes are
marked by white arrowheads (K) and one is enlarged in L. (M) The uterus of a
WEE-1.3-depleted animal in which the oocyte chromosomes have begun to
endoreplicate. ut, uterus; spth, spermatheca. Scale bars: in A, 20 µm for
A,C,F,G,I,K,M; in B, 5 µm for B,D,H,J,L; in E, 5 µm.
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