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Figure 8


Fig. 8. The chromosome coalescence phenotype of WEE-1.3-depleted oocytes is dependent on the presence of sperm. Diakinetic chromosomes (arrows) of oocytes in the proximal gonad are evident in DAPI-stained wild-type hermaphrodites (A), in unmated (C) or mated (D) fog-2 females, and in unmated WEE-1.3-depleted fog-2 females (E). Although the oocytes in fog-2 animals remain in diakinesis (C-E), the chromosomes of WEE-1.3-depleted fog-2 oocytes coalesce into one mass upon the introduction of sperm (F). This phenotype appears identical to that observed in wild-type hermaphrodites depleted of WEE-1.3 (B). The white carets (^) mark the oocyte chromosomes that have coalesced (B,F). The spermatheca (spth) is to the left in each panel. Scale bar: 20 µm.





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