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Figure 3


Fig. 3. CG5175/char is required for nuclei organisation and apical anchoring. (A,B) Phase-contrast views of living Drosophila embryos at different time intervals. In a control embryo (A), the nuclei (white) elongate and keep a regular organisation until gastrulation (arrow). In a char RNAi embryo (B), the nuclei progressively lose their proper apical alignment when the membrane invagination front reaches the basal extent of the nuclei (top, middle and arrows in insets) and acquire an irregular round morphology later (arrow). Blue arrowheads indicate the position of the membrane invagination front in the insets. (C,D) Confocal images at successive time points of cellularisation in control embryos (C) and char RNAi embryos (D). Nuclei are labelled with Hoechst (green) and PatJ (blue) highlights the membrane invagination front. (E,F) Nuclear envelope (marked with a Lamin antibody, red) and nucleus (green) in control (E) and char RNAi embryos (F) at early and late stages of cellularisation and viewed from the top.





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