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Figure 2


Fig. 2. bel mutations disrupt zebrafish lhx2. (A) Map of the bel genetic region on chromosome 8. The number of recombinants for each marker is shown. BAC clones linked to bel are shown below the line. (B) Sequence comparison of Lhx2 protein sequence from zebrafish, chick, and mouse. Underlined sequence represents the two Lim domains; boxed sequence is the conserved homeobox domain. (C) Cladogram showing sequence divergence of Lhx2 and the closely related family member (Lhx9, Genbank Accession number NM001017710) in zebrafish, chick and mouse. (D) Sequence defects lead to premature stop codons in both bel alleles. In belb700, a 22 bp deletion starting at position 664 (underlined in wild-type sequence) leads to a frame-shift and introduces a premature stop codon following four novel amino acids. In beltv42, a transversion (C to A) introduces a premature stop codon at position 243. (E) Schematic of the Lhx2 protein showing LIM domains (green), the homeobox domain (blue), intron/exon borders (black lines), and the position of the premature stop codons encoded by the two bel alleles (arrowheads).





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