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Figure 1


Fig. 1. Expression of {alpha}-cardiac actin in cells of the dorsal aorta, visualized as ß-gal activity with the T4 transgenic mouse line. (A) Whole-mount X-gal staining of an E10.5 embryo, showing ß-gal labelling (blue) in the heart (H), somites (S) and dorsal aorta (DA). FL, forelimb; HL, hindlimb. (B) A ventral view of A. (C) Immunohistological staining with an {alpha}-smooth muscle actin antibody (red) showing labelled cells in the dorsal aorta (DA) and myotomes (M), on either side of the neural tube (NT). (D) Co-immunohistology of the section shown in C, stained with antibodies to ß-gal (green) and {alpha}-smooth muscle actin (red). (E) An enlargement of the dorsal aorta (DA), showing co-immunohistological staining with antibodies to {alpha}-smooth muscle actin (red) and ß-gal (green). White arrowheads show {alpha}-smooth muscle actin-positive cells that have ß-gal+ nuclei and are in a periendothelial position. The white arrow points to a vascular smooth muscle cell that is also ß-gal+. (F) The same view with DAPI staining (blue) shows the nuclei of ß-gal+ smooth muscle cells (white arrowheads and white arrow) and adjacent endothelial cells (grey arrowheads). (G) Co-immunohistological staining with anti-laminin (red) and anti-ß-gal (green) antibodies shows a cell (white arrowhead) with a ß-gal+ nucleus in the pericyte position within the basal lamina which underlies the endothelial cell layer of the dorsal aorta (DA) (H) The same section with DAPI staining shows the position of this cell (white arrowhead) immediately below an endothelial cell (grey arrowhead) which faces into the lumen of the dorsal aorta (DA). (I) Co-immunohistological staining with antibodies to the endothelial specific marker CD31/PECAM (red) and ß-gal (green). CD31-positive endothelial cells that also express ß-gal are marked by white arrowheads. Other cells with ß-gal+ nuclei (green) in the dorsal aorta (DA) are not endothelial. NT, neural tube. (J) The same view with DAPI staining showing the endothelial position of the nucleus adjacent to the lumen (white arrowheads) of the ß-gal/CD31-positive cells. (K) Whole-mount X-gal staining of an E9.5 embryo, showing ß-gal labelling in the heart (H) and somites (S). FL, forelimb bud. (L) A ventral view of K shows labelled cells in the dorsal aorta (DA). Scale bars: 20 µm.





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