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Fig. 4. Role of HBEGF shedding at 2% O2. (A) Cellular and
secreted HBEGF concentrations were measured by ELISA after culture for 8 hours
at 2% O2 in medium containing the indicated concentrations of
GM6001. HBEGF values were normalized to vehicle, as in
Table 1.
*P<0.05 compared with vehicle. (B)
Cytotrophoblast cells were cultured for 8 hours at ambient O2
levels (black bars) or 2% O2 (gray bars) in medium supplemented
with vehicle, 10 µg/ml GM6001 or 10 µg/ml of an inactive structural
analog (Control) and TUNEL was determined. *P<0.05
compared with vehicle treatment. (C) Cells were cultured for 8 hours at
2% O2 with 0 µg/ml (x) or 10 µg/ml (all other symbols)
GM6001 and apoptosis was determined by TUNEL. The indicated concentrations of
recombinant human HBEGF (black squares) were added to GM6001-treated cells.
Co-treatment was also conducted with 10 nM of other recombinant EGF family
growth factors, including epiregulin (open circle), betacellulin (open
diamond), amphiregulin (open triangle), EGF (open square) or TGF
(solid
triangle). The 0 µg/ml GM6001 control was different (P<0.05)
from all other treatments except those containing 10 µg/ml GM6001 plus 1-10
nM HBEGF.
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