QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Requirement of Shh forebrain enhancers in the context of Bac 447L17ßlacZ. Schematic representation of the SBE2, SBE3 and SBE4 deletion constructs. X-gal staining in the forebrain of embryos carrying (A-D) 447L17ßlacZ^SBE2, (E-H) 447L17ßlacZ^{SBE2, SBE3}, (I-L) 447L17ßlacZ^SBE4, (M-P) 447L17ßlacZ^{SBE3, SBE4}, (Q-T) 447L17ßlacZ^{SBE2, SBE4} and (U-X) 447L17ßlacZ^{SBE2, SBE3, SBE4}. Deletion of SBE2 resulted in the loss of reporter activity at most levels of the diencephalon, including the preoptic area (POA; arrowhead in D). Compare A-D with the pattern of X-gal staining in embryos carrying the wild-type 447L17ßlacZ transgene (Fig. 3B,H,N,T). Embryos carrying 447L17ßlacZ^{SBE2, SBE3} (E-H) showed patterns of X-gal staining that were similar to those carrying 447L17ßlacZ^SBE2 (A-D). Embryos carrying 447L17ßlacZ^SBE4 (I-L) showed an absence of staining in the ventricular zone (vz) of the mge (L). In embryos carrying 447L17ßlacZ^{SBE3, SBE4} (M-P), X-gal staining was not detected in the vz or subventricular zone (svz) of the medial ganglionic eminence (mge) (P). Deletion of SBE2 and SBE4 resulted in a loss of expression in the vz of the mge and the entire diencephalon (Q-T). Embryos carrying 447L17ßlacZ^{SBE2, SBE3, SBE4} (U-X) showed no expression in the diencephalon or telecephalon. The ratio of embryos exhibiting reproducible Shh-like reporter activity over the total number of transgenic embryos is indicated for each construct (A,E,I,M,Q,U).

Return to article