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Figure 5


Fig. 5. Conservation of SBE2 sequence and function. (A) Vista plots comparing the alignment of human SBE2 sequences with mouse, chicken, frog and tetraodon. (B) Comparison of the distance between SBE2 and the Shh transcription start site in human, mouse, chicken, frog and tetraodon. (C-F) SBE2 reporter activity derived from (C) human, (D) chicken, (E) frog and (F) pufferfish sequences. X-gal staining in the otic vesicle of the embryo shown in F is ectopic. The ratio of embryos exhibiting reproducible Shh-like reporter activity over the total number of transgenic embryos is indicated for each construct (C-F).





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