DEV02253 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Elav expression in assembling domain is abolished in egfr mutant clones but is not affected in sim mutant brains. (A-C) egfr mutant clones abolish Elav expression cell autonomously. Clones are visualized by the absence of GFP (A; green), and are outlined in A and B. Elav expression is visualized with an anti-Elav body (B,C; magenta). (D-I) Cells in the assembling domain in sim²/sim^ry75 mutant brains express Elav (magenta). Control (D-F) and sim²/sim^ry75 mutant (G-I) brains are shown. Dac expression (cyan in D,G) and R axons (white in D,E,G,H) are visualized with respective antibodies.

• Supplemental Figure 2 -

  Fig. S2. Cell division patterns are not affected in sim loss-of-function mutant brains but are affected in sim overexpressing brains. (A-F) Cells in G1-S transition phase were marked by anti-E2F detection (magenta). Lamina neurons were marked by Dac (cyan in A-C). (A,D) E2F expression patterns in control brains. (B,E) Overexpression of sim in lamina neurons using NP6099-GAL4. E2F expression at the lamina furrow is abolished in sim overexpressing brains (arrowhead in E). (C,F) E2F expression pattern is not affected in the sim²/sim^ry75 mutant lamina. E2F signal is greatly reduced in the mutant inner proliferating center (arrow).

• Supplemental Figure 3 -

  Fig. S3. Sim expression in lamina neurons is not affected in dac mutant clones. (A-C) Clones are visualized by the absence of GFP (A,B; green). Sim expression is visualized with an anti-Sim antibody (A,C; magenta). A merged image of A and B is shown in C. dac mutant clones normally express Sim.