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Figure 2


Fig. 2. Development of lamina neurons and R axon projection. (A-H) Positional relationship between R axons and lamina neurons in early to mid third instar larval brains. Single optical sections (A-D) and 3D reconstructions (E-H) of distinct developmental timing through the third instar larval stage. Lamina neurons visualized by Dac (magenta) and R axons visualized by anti-HRP (white) are shown. In early stages of four to five (A,E), six to seven (B, F) and 11 to 12 (C,G) rows of ommatidial differentiation, lamina neurons are not present in the assembling domain (encircled by yellow dots in F,G). Lamina neurons are detected in the assembling domain when 13 to 14 rows of ommatidia have differentiated (D,H), and at all later stages. (I-K') Newly arriving axons make contact with existing axons. R axons are visualized by GMR-GAL4 UAS-GFP (green), whereas only anterior-most (most recently arriving) R axon bundles are detected by anti-Flamingo antibody (magenta). Images are from a lateral perspective (I,J,J') and a horizontal perspective (K,K'). A magnified image of the dorsal area (white-lined box) in I is shown in J with Fmi signal, and in J' without Fmi signal. Fmi-positive R axon bundles (arrow in K) make contact with Fmi-negative bundles (arrowhead in K) all along the entire lamina layer.





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