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Fig. 4. Single-minded is specifically expressed in lamina under the control of
Hh signaling. (A,B) Single-minded (Sim) is expressed specifically
in lamina neurons. Sim (white) and Dac (blue) protein are visualized by
immunofluorescence microscopy. (C) A magnified image of the boxed area
of B. Nuclei of the lamina neurons were visualized by NP6099-GAL4
UAS-nlsGFP in C'. Note that nuclear localization of Sim is obscure
in the anterior (left side) and obvious in the posterior (right side) region.
(D) A marginal level of Sim expression (white) is detected in the eye
disc. Dac (blue) is expressed at the morphogenetic furrow. (E-G) Sim
expression is cell-autonomously abolished in smo mutant clones.
smo mutant clones (arrowheads) are visualized by the absence of GFP
(green); Sim (magenta) and Dac (blue in E; cyan in G) were visualized by
immunofluorescence microscopy.
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