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Fig. 5. sim mutant lamina neurons are not incorporated into the assembling domain. (A-C) sim² clones are not recovered in the assembling domain. Clones are visualized by the absence of GFP (green in A,B), R axons by anti-HRP (white) and lamina neurons by anti-Dac (blue in the merged image B). Mutant clones in lamina are shown in C (yellow). (D-M) Fewer lamina neurons are incorporated into the assembling domain in the mutant. Wild-type (D-F) and sim²/sim^ry75 mutant (G-I) lamina neurons are marked by Dac (magenta in D,F,G,I) and R axons are visualized by anti-HRP (white in D,E,G,H). 3D reconstructions of Dac expression in D and G are shown in F and I, respectively. In contrast to wild type (D-F), in sim²/sim^ry75 mutant brains, a smaller number of lamina neurons are present in the assembling domain (compare G with D, arrows), leaving more lamina neurons behind in the pre-assembling domain (compare G with D, arrowheads). In sim²/sim^ry75 mutant brains, the assembling domain is smaller (H) than in wild type (E). (J-M) Wild-type (J) and sim²/sim^ry75 mutant (L) lamina viewed from a horizontal perspective. Dac expression (magenta) and R axons (white) are visualized with the respective antibodies. Assembling domains are encircled by a yellow line. The number of lamina neurons in the assembling domain is reduced (arrows in J,L) and the number of lamina neurons in the pre-assembling domain is increased (arrowheads in J,L) in sim²/sim^ry75. (K,M) Schematic illustration of J and L, respectively. Colors of cells are as noted in Fig. 1C.

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