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Fig. 6. Targeted expression of dominant-negative dARNT prevents lamina neurons
from assembling lamina columns. (A-C) NP6099-GAL4
expression in the optic lobe visualized using UAS-nlsGFP (green) and
anti-Dac (magenta in merged image B). NP6099-Gal4 is also expressed
in a small part of the most posterior eye disc, including in about 10
ommatidia (arrowhead in C). R axons are visualized by 24B10 (magenta in C).
(D-F) A dominant-negative form of dARNT inhibits the incorporation of
lamina neurons into the assembling domain; Dac, magenta and R axons, white. 3D
reconstruction of Dac expression in D is shown in F. Lamina neurons are almost
never seen in the assembling domain (F, arrow in D). (G-I) Effect of a
dominant-negative form of dARNT expression is suppressed by co-expression of
sim; Dac, magenta and R axons, white. 3D reconstruction of Dac
expression in G is shown in I. Lamina neurons are incorporated in the
assembling domain (arrow in G) and lamina columns as in the wild type.
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