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Fig. 7. Overexpression of sim causes the premature incorporation of
lamina neurons into the assembling domain. (A-C) Overexpression of
sim in lamina neurons using NP6099-GAL4 (B,C). Lamina
neurons are marked by Dac (magenta) and R axons are visualized by anti-HRP
(white in A,B). NP6099-GAL4 driving GFP alone was used as a
control (A). 3D reconstruction of Dac expression in B is shown in C. The
number of lamina neurons in the pre-assembling domain is reduced (arrowheads
in A-C). (D-I) Horizontal views of wild-type and mutant lamina at third
larval instar. In contrast to wild type (D,G) and sim mutants (F,I),
lamina neurons overexpressing sim (E,H) do not migrate apically;
instead, they enter the assembling domain directly (arrowheads in D-F; yellow
broken lines in G-I). The lamina precursor cells at the lamina furrow are
visualized with tkv-lacZ (magenta). Lamina neurons are marked by
NP6099-GAL4 UAS-nlsGFP (blue in D) or Dac (blue in E,F), and R axons
by anti-HRP (white). G-I are single channel of tkv-lacZ for D-F,
respectively. The migratory route is shown by arrows.
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