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Fig. 1. cvl2 is co-expressed with bmps and positively
regulated by Bmp signaling. (A) Domain composition of Cvl2 compared
with the CR domain proteins Chordin (Sasai
et al., 1994), Neuralin
(Coffinier et al., 2001) and
Kielin (Matsui et al., 2000).
Blue boxes indicate signal peptide; red boxes indicate CR domains; yellow
boxes indicate vWFd domain; gray box indicates the Thrombospondin domain;
numbers one the right indicate total number of amino acid residues. (B)
Temporal expression profile of cvl2 determined by RT-PCR.
cvl2 is only zygotically expressed. (C-K) Spatial expression
pattern determined by in situ hybridization; developmental stage is indicated
in lower left-hand corner; genotype of embryos is indicated on the right-hand
side. (C-E) Lateral views, ventral leftwards. (F,H) Frontal views, focal plane
of H is indicated in G. (G) Animal pole view on head, ventral leftwards.
Filled arrows in E-H indicate anterior lateral mesoderm; open arrows in D,F
indicate prechordal plate; arrowhead in E indicates ventral tailbud mesoderm.
(I-K) Vegetal views, ventral leftwards, with dorsal borders of the expression
domain indicated by arrows. (I,J) cvl2 expression is expanded
dorsally in chordin mutants. (I,K) Ventral expression is absent in
bmp2b mutants. (L-N) Late cvl2 expression at 34 hpf
in otic vesicle (arrowhead in L) and pharyngeal pouches (arrows in L) is
elevated in hsp70::bmp2b transgenic (M), but downregulated in
hsp70::noggin3 transgenic (N) embryos after a 30 minutes heatshock
(28°C to 39°C) at 26 hpf.
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