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Fig. 4 . Caspase 3 and caspase 6 immunostaining. Immunostaining of the
anterior region of secondary lens fiber cells (A) in wild-type
(B,D,F,H) and DKO (C,E,G,I) lenses with anti-active caspase 3
(B,C) and anti-caspase 6 (F,G) antibodies. In negative controls (D,E,H,I),
primary antibodies were omitted and only the secondary anti-rabbit IgG
antibody, conjugated with Alexa Fluor 488, was used. (J) Image-Pro Plus
v.5.1 software was used to quantitate fluorescence intensity in sections of
lens from wild-type and DKO mice. Scale bar: 25 µm.
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