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Figure 5


Fig. 5. TUNEL labeling of lens secondary fiber cells. Bright-field image (A-D), TUNEL reaction fluorescence (E,F) and merge of both (G,H) sections from 7-week-old wild-type (A,C,E,G,I) and 7.5-week-old DKO (B,D,F,H,J) mouse lenses. In negative controls (I,J), calf thymus terminal deoxynucleotidyl transferase was omitted from the TUNEL reaction. C,D are enlargements of boxed areas in A,B. Arrows in E,F,H indicate TUNEL-positive nuclei. Scale bar: 125 µm in A,B; 25 µm in C-J.





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