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Figure 2


Fig. 2. Expression patterns of Robo1 and Robo2 during mammary gland development. (A,B) ROBO1 immunostaining on (A) +/+ and (B) Robo1-/- EBs. (C,D) Robo1-/- outgrowth stained for ß-galactosidase activity in the (C) EB and (D) duct. (D) A grazing longitudinal section through a duct. Arrowheads identify examples of MECs co-expressing Robo1 (blue) and SMA (brown). (E) RT-PCR using Dutt1- and Robo1-specific primers. Lane 1, P4 cerebellum (control); lane 2, mammary gland. A 100 bp ladder (NEB) was used as a marker, as shown on the left-hand side. Dutt1 generates a 571 bp PCR fragment and Robo1 generates a 428 bp PCR fragment. (F,G) Robo2+/- glands stained for ß-galactosidase activity in the mature virgin duct (F) and aveoli during pregnancy (G). Arrowheads indicate examples of positively stained cells in the cap cell layer of the EB (A,C) and MEC layer of the duct (D,F). (G) Arrows indicate examples of positively stained Robo2-expressing cells. L, lumen. Scale bar: 10 µm.





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