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Fig. 1. Ventral tangential cell migration in organotypic culture. (A)
Schematics of organotypic culture. A strip was dissected from the E10.5 mouse
telencephalon along the dorsoventral axis to include the presumptive LOT area
(green area) around the middle. A small area in the dorsal neocortex of the
strip was labeled with fluorescent dye rhodamine (asterisk). (B) The
strip 3 hours after preparation. Rhodamine-labeled cells (magenta) do not
migrate yet from the labeled position (asterisk). Boundary between the
neocortex and the GE is visualized by immunostaining against reticulon 1
(green). (C) The strip after 36 hours in culture. Merged image of
rhodamine (magenta) and immunostaining with mAb lot1 (green). One of the
rhodamine-labeled cells migrating ventrally reacts with mAb lot1 (arrowhead).
(D,E) The strip after 2 days in culture. Many rhodamine-labeled cells
(magenta in D, white in E) migrate in the ventral direction from the dye
injection point (asterisk) and stop before entering the GE. Reticulon 1 is
immunostained in green in D. (F,G) High-magnification views of the
upper (F) and lower (G) boxes in E. The processes of many rhodamine-labeled
cells are directed in the ventral direction in F, but reoriented in the
rostrocaudal direction at the presumptive LOT area in G. Left, rostral aspect;
top, dorsal aspect; dcx, dorsal neocortex; vcx, ventral neocortex; GE,
ganglionic eminence. Scale bars: 500 µm in B,D; 50 µm in C,F,G.
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