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Fig. 2. Tangential cell migration in organotypic co-culture. (A)
Combination of the dorsal neocortex from a green mouse embryo with the ventral
neocortex in a wild-type strip in the dorsoventrally ordered manner. Many
GFP-expressing cells migrate through the ventral neocortex down to the
presumptive LOT area. (B) Combination of the wild-type dorsal neocortex
with the ventral neocortex in a green mouse strip in the dorsoventrally
ordered manner. GFP-expressing cells do not migrate into the dorsal neocortex.
(C) The isolated dorsal neocortex labeled with rhodamine (asterisk) and
cultured alone for 2 days. Rhodamine-labeled cells migrate ventrally and
accumulate in the ventral edge of the explant. (D) Co-culture of two
dorsal neocortices ventrally facing each other, one of which was injected with
rhodamine (asterisk). Rhodamine-labeled cells migrate and stop close to the
combination boundary with the other explant. (E) Co-culture in which
the dorsal neocortex of a green mouse embryo was combined with the lateral
side of a wild-type strip. GFP-expressing cells preferentially penetrate in
the LOT area and the neocortex but not the GE. (F,G) The dorsal
neocortex prepared from a green mouse embryo (F) or labeled with rhodamine (G)
was combined with the ventral side of the GE in a wild-type strip in the
dorsoventrally reversed orientation. In neither of the co-cultures did cells
of the combined dorsal neocortex penetrate into the GE. (H)
High-magnification view around the combination boundary in G.
Rhodamine-labeled cells perpendicularly change orientation and align along the
boundary. Broken white lines outline the explants and broken green lines
indicate the combination boundaries in A-H. The ventral direction of each
explant is indicated by an arrow. (I) A possible scheme of regulation
of the ventral tangential migration. The neocortex contains ventrally
directing signals for migrating cells. The whole area of the GE has mechanisms
to exclude lot cells. Scale bars: 500 µm in A-G; 100 µm in H.
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